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aphanomyces-featured-v2
May 21, 2025

Aphanomyces

20/20 Seed Labs Disease

 

An article from Trevor Blois, Disease Diagnostician on the pathogen – Aphanomyces

Root rot in pulse crops is a major concern for growers. It can be caused by a number of pathogens including Fusarium species, Pythium species, and Rhizoctonia solani. Since 2012, however, a new pathogen has been the biggest root rot concern in western Canada: Aphanomyces euteiches.

Just like any root rot pathogen, infection by Aphanomyces causes damage to roots, interfering with a plants’ ability to uptake nutrients and water, as well as reduce nodulation. This will manifest above-ground as chlorotic, stunted plants, with significantly reduced yields. Infection is particularly favoured by wet and warm conditions.

There are several reasons that Aphanomyces is a different beast from the other root rot pathogens. Aphanomyces is the most destructive disease of peas, especially when other root rot pathogens are present. Infected roots show characteristic caramel colouring that are very soft when compared with healthy roots. These roots produce abundant thick-walled spores, called oospores, which can be easily spread through the movement of soil. Another reason Aphanomyces is a particular concern is that these spores are very long lived, so if Aphanomyces is detected in a field, crop rotations are recommended to be extended up to eight years between hosts to reduce inoculum levels.

When Aphanomyces was first found in Western Canada there were no registered seed treatments. Now Intego Solo, Rancona Trio, and Zeltera Pulse all offer suppression of early season Aphanomyces. Rancona Trio can also be mixed with Belmont or Intego Solo for control of early season Aphanomyces. These seed treatments will only reduce risk of infection early in the season. If disease pressure is very high or conditions are more favourable for infection later in the season seed treatments may offer little protection.

To protect your crop from Aphanomyces there are several steps that you can take:

  • Scout and test suspect plants. Any plants suspected of Aphanomyces infection can be sent into the lab to test for which root rot pathogens are present. If Aphanomyces is confirmed, extend the rotation to 6 to 8 years between host crops, peas and lentils. Avoid moving soil from the infected field to other fields that have peas or lentils in them the following year.
  • Test soil if you are planning on planting peas or lentils into a field, and if it’s positive choose a non-host crop to seed instead. To test soil, sample in a W-shaped pattern across the field, or focus on low spots, water runs, compacted areas where the pathogen is most likely to be found. Clear all loose organic matter from the soil surface and collect the top 5-10 cm of the A-horizon, or less as the depth allows, without taking any of the B-horizon. Submit a minimum 2 cup sample of soil. Air dry and send in a Ziploc freezer bag.
  • Test your seed and only use seed with high levels of germination and vigour, and low levels of seed-borne pathogens. High quality seed will reduce the stressors, ensure better establishment, giving the plants the best chance to fight off an infection if conditions for disease become favourable.
  • Apply a seed treatment with Aphanomyces suppression on the label, these will help to prevent early season infections, giving the plants a better start.

Aphanomyces is widespread in the prairies, so if you are a pulse grower you may not be able to avoid it, but by following these precautions you may be able to lessen the impact of this devastating disease.

Schedule a quick chat with Trevor Blois, Disease Diagnostician

https://2020seedlabs.ca/contact-us/

Learn more about 20/20 Seed Labs Inc. Testing Services

https://2020seedlabs.ca/services/

Why Smart Businesses Start with the Right Questions—and the Right Lab Food for Thought: The Times They Are a-Changin`

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2020 Seed Labs

Contact Us

support@2020seedlabs.ca

Manitoba
3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Phone: 1-204-261-3755

Alberta
507 – 11 Avenue
Nisku, AB T9E 7N5
Phone: 1-877-420-2099

© Copyright 20/20 Seed Labs Inc. 2024. All rights reserved. Privacy Policy
  • 1-877-420-2099
  • support@2020seedlabs.ca

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How to Submit a Sample
to 20/20 Seed Labs Inc.

  Please use our our ‘3-Step Process’ when submitting samples to be tested.

Take a Representative Sample

Select Your Testing and Enter Your Sample Details

Ship or Drop Off Your Sample To Our Lab

STEP 1: Take a Representative Sample

We provide testing on seeds, plants and soil. Select the type of sample below that you are submitting for sample collection instructions.

  • Seed Samples

    The table below outlines the type of seed test and required sampling amount needed for each test. Be sure to follow the 4 steps indicated to collect your sample.

    Cereals

    Seed Health & Disease Tests
    500 g (2 1/2 cups)
    Purity Tests
    1 kg (5 cups)

    Pulses

    Seed Health & Disease Tests
    500 g (3 cups)
    Purity Tests
    1 kg (6 cups)

    Grasses & Forages

    All Testing
    250 g

    Canola

    All Testing
    250 g
    20/20 Seed Labs - How to Take a Seed Sample
  • Plant Samples

    The sampling cards below outline the steps to collect your required plant samples for testing.



  • Soil Samples

    The sampling cards below outline the steps to collect your required soil samples for testing.


STEP 2: Select Your Testing and Enter Your Sample Details

Select the testing services you will require from the pricing schedule below, then choose how you want submit your sample: 

Option 1:
Submit your sample online: 

Option 2:
Write Your customer number and sample details on the sample bag and prepare the sample for shipping or drop-off.

STEP 3: Ship or Drop Off Your Sample to Our Lab

Mailing Addresses

NISKU, ALBERTA

20/20 Seed Labs, Inc.
507-11 Avenue
Nisku, AB T9E 7N5
Toll Free: 1-877-420-2099

WINNIPEG, MANITOBA

20/20 Seed Labs, Inc.
3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Toll Free: 1-866-540-7333

After Hours Drop Off

There is a locked and secure after hours drop off box in front of both the Nisku, Alberta and Winnipeg, Manitoba 20/20 Seed Labs offices. Please leave your samples in the drop off box if you are dropping off a sample on the weekends, a statutory holiday, or after 4:30 p.m. on a weekday.

Sample Drop Off Locations

agroplus-logo

Agro Plus Inc.

3895-9th Avenue North
Lethbridge, Alberta
T1H 6G8
Phone: 1-403-327-7070

down-to-earth-labs-logo

Down to Earth Labs

3510-6th Avenue North
Lethbridge, Alberta
T1H 5C3
Phone: 1-403-328-1133

ici-logo

Independent Crop Inputs Inc.

Hwy #3, 2.5km East of Barnwell, Alberta
Box 4690, Taber, Alberta
T1G 2E1
Phone: 1-403-223-1325

  • Step 1: Review Requirements

    Requirements for Submitting Samples

    Please submit the correct sample size for the test required.

    Germination and Vigour Tests
    Most tests of this type require approximately 250 grams or 1 cup of seed. For very large seeds such as some types of peas or beans, up to twice as much seed may be required.

    Disease and Seed Health Tests
    The quantity of seed for most disease testing is the same as a germination test. Some tests with special requirements may require larger amounts of seed.

    Purity Tests
    Test volumes for purity and % pure seed vary with crop type and are available on the purity weight form.

    Rhizobia Test
    Small seeded legumes require a 1 cup sample. Large seeded legumes require a minimum 2 cup sample. Inoculant, loose peat, granules, and liquid  should be tested in the original sealed container size.

    Canadian Seed Institute Samples
    Follow the CSI instructions for the crop type and the weight to be submitted. Example: Wheat, Purity = a minimum of 1 kg of seed.

    APHIS Samples
    Quantities of seed to be tested for an APHIS certificate for export to the US vary with crop type. Please contact us for the correct volume required.

    Soil Samples
    We test soil for clubroot and for a weed check analysis. It is preferable to have a minimum 2 cup or 1 kg sample size.

    Plant Specimens
    Root samples for clubroot analysis should have only one plant per bag and other plant or leaf samples should contain an adequate amount for representation of the tissue. Please pack fresh samples with moist paper towel and drop off at lab or ship by overnight courier only. Pack in a cooler with ice packs if possible. Do not allow sample to contact ice directly.

    FLAX / TRIFFID Tests
    This test requires either 1 or 2 kg of seed. Please CLICK HERE to use the FLAX SAMPLE SUBMISSION FORM for all samples and to confirm the exact amount of seed required.

    If you’re still unsure of the sample size required for your test, please contact us.

  • Step 2: Packaging

    Packaging

    Printed Sample Bags

    20/20 Seed Labs Inc. provides printed sample bags to all of our customers so they may submit samples with all the information clearly noted. As well, you can use packaging or bags from other labs if necessary.

    Special Requirements

    Some samples require special packaging to ensure the sample’s integrity is not compromised during shipping. Please determine if your sample may require any of the following procedures to keep the sample in proper condition.

    Ice Packs

    For samples that need to remain cool during shipping, such as seed or inoculant samples for rhizobia testing.

    Heavy-duty Sealed Poly Bags

    Samples requiring moisture testing should be in a container that will reduce the risk of moisture loss that could cause a change in the sample quality from time of shipping to the time of testing.

  • Step 3: Submit Your Sample

    Guide to Submitting Your Sample Online

    1. Login or sign up for a 20/20 account
    2. Click the “create sample” button
    3. Pick a crop
    4. Choose the number of similar samples for a crop
    5. Enter the information about the seed lot(s); lot numbers, sampler, processor, etc.
    6. Enter the packages or tests you require. (Click here to download a list of our testing packages)
    7. Choose method of printing a bar-coded label (Either a small self-adhesive label or a regular paper label)

    LOG IN NOW | CREATE ACCOUNT

    Other Submission Options

    Submit Your Sample by Mail

    20/20 Seed Labs Inc. receives shipments from all couriers, bus and mail services on a daily basis. You should determine the best method of shipping your samples to ensure it meets your requirements for timeliness and sample integrity upon arrival. We process all samples on arrival and customers with a user name and login can monitor the status of their sample online to determine when it arrived, estimated completion date and check test results.

    Submit Your Sample In-Person

    You may bring your samples directly to our lab, or the after hours drop-off slot. Please call if you have any questions:

    Toll Free: 1-877-420-2099

    In Alberta:
    20/20 Seed Labs Inc.
    507 – 11th Ave
    Nisku, Alberta, Canada
    T9E 7N5

    In Manitoba:
    20/20 Seed Labs Inc.
    3489 Pembina Hwy
    Winnipeg, MB R3V 1A4
    Phone: 1-204-261-3755

X

STEP 3: Ship or Drop Off Your Sample to Our Lab

Mailing Addresses

NISKU, ALBERTA

20/20 Seed Labs, Inc.
507-11 Avenue
Nisku, AB T9E 7N5
Toll Free: 1-877-420-2099

WINNIPEG, MANITOBA

20/20 Seed Labs, Inc.
3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Toll Free: 1-866-540-7333

After Hours Drop Off

There is a locked and secure after hours drop off box in front of both the Nisku, Alberta and Winnipeg, Manitoba 20/20 Seed Labs offices. Please leave your samples in the drop off box if you are dropping off a sample on the weekends, a statutory holiday, or after 4:30 p.m. on a weekday.

Sample Drop Off Locations

agroplus-logo

Agro Plus Inc.

3895-9th Avenue North
Lethbridge, Alberta
T1H 6G8
Phone: 1-403-327-7070

down-to-earth-labs-logo

Down to Earth Labs

3510-6th Avenue North
Lethbridge, Alberta
T1H 5C3
Phone: 1-403-328-1133

ici-logo

Independent Crop Inputs Inc.

Hwy #3, 2.5km East of Barnwell, Alberta
Box 4690, Taber, Alberta
T1G 2E1
Phone: 1-403-223-1325

  • Step 1: Review Requirements

    Requirements for Submitting Samples

    Please submit the correct sample size for the test required.

    Germination and Vigour Tests
    Most tests of this type require approximately 250 grams or 1 cup of seed. For very large seeds such as some types of peas or beans, up to twice as much seed may be required.

    Disease and Seed Health Tests
    The quantity of seed for most disease testing is the same as a germination test. Some tests with special requirements may require larger amounts of seed.

    Purity Tests
    Test volumes for purity and % pure seed vary with crop type and are available on the purity weight form.

    Rhizobia Test
    Small seeded legumes require a 1 cup sample. Large seeded legumes require a minimum 2 cup sample. Inoculant, loose peat, granules, and liquid  should be tested in the original sealed container size.

    Canadian Seed Institute Samples
    Follow the CSI instructions for the crop type and the weight to be submitted. Example: Wheat, Purity = a minimum of 1 kg of seed.

    APHIS Samples
    Quantities of seed to be tested for an APHIS certificate for export to the US vary with crop type. Please contact us for the correct volume required.

    Soil Samples
    We test soil for clubroot and for a weed check analysis. It is preferable to have a minimum 2 cup or 1 kg sample size.

    Plant Specimens
    Root samples for clubroot analysis should have only one plant per bag and other plant or leaf samples should contain an adequate amount for representation of the tissue. Please pack fresh samples with moist paper towel and drop off at lab or ship by overnight courier only. Pack in a cooler with ice packs if possible. Do not allow sample to contact ice directly.

    FLAX / TRIFFID Tests
    This test requires either 1 or 2 kg of seed. Please CLICK HERE to use the FLAX SAMPLE SUBMISSION FORM for all samples and to confirm the exact amount of seed required.

    If you’re still unsure of the sample size required for your test, please contact us.

  • Step 2: Packaging

    Packaging

    Printed Sample Bags

    20/20 Seed Labs Inc. provides printed sample bags to all of our customers so they may submit samples with all the information clearly noted. As well, you can use packaging or bags from other labs if necessary.

    Special Requirements

    Some samples require special packaging to ensure the sample’s integrity is not compromised during shipping. Please determine if your sample may require any of the following procedures to keep the sample in proper condition.

    Ice Packs

    For samples that need to remain cool during shipping, such as seed or inoculant samples for rhizobia testing.

    Heavy-duty Sealed Poly Bags

    Samples requiring moisture testing should be in a container that will reduce the risk of moisture loss that could cause a change in the sample quality from time of shipping to the time of testing.

  • Step 3: Submit Your Sample

    Guide to Submitting Your Sample Online

    1. Login or sign up for a 20/20 account
    2. Click the “create sample” button
    3. Pick a crop
    4. Choose the number of similar samples for a crop
    5. Enter the information about the seed lot(s); lot numbers, sampler, processor, etc.
    6. Enter the packages or tests you require. (Click here to download a list of our testing packages)
    7. Choose method of printing a bar-coded label (Either a small self-adhesive label or a regular paper label)

    LOG IN NOW | CREATE ACCOUNT

    Other Submission Options

    Submit Your Sample by Mail

    20/20 Seed Labs Inc. receives shipments from all couriers, bus and mail services on a daily basis. You should determine the best method of shipping your samples to ensure it meets your requirements for timeliness and sample integrity upon arrival. We process all samples on arrival and customers with a user name and login can monitor the status of their sample online to determine when it arrived, estimated completion date and check test results.

    Submit Your Sample In-Person

    You may bring your samples directly to our lab, or the after hours drop-off slot. Please call if you have any questions:

    Toll Free: 1-877-420-2099

    In Alberta:
    20/20 Seed Labs Inc.
    507 – 11th Ave
    Nisku, Alberta, Canada
    T9E 7N5

    In Manitoba:
    20/20 Seed Labs Inc.
    3489 Pembina Hwy
    Winnipeg, MB R3V 1A4
    Phone: 1-204-261-3755

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