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20230726_114710
August 14, 2023

Vigour Testing – The Plots Told the Tale

20/20 Seed Labs Agronomy, Seed Health

By Shanyn Silinski, Marketing Manager 

This spring Rutherford Farms (thanks, team Rutherford, you’ve been great!) planted a series of soybean plots. They were all from the same variety but had different results on their vigour tests and similar germination numbers. They were labelled: Control, Train Wreck, Low, Moderate and High Vigour. 

The differences were notable, even in what most consider a very optimal planting with good soil temperatures and moisture. 

 Sarah Foster presented at the Rutherford Farms Field Day about the test results, the plots and spoke about the planting, the conditions, and what she saw in each plot and how it spoke to the importance of vigour testing.

Emily Laudin, Sales Agronomist and Rutherford Farms Host, with Sarah Foster, President and Senior Analyst 20/20 Seed Labs, talking to the Field Day guests about the textbook vigour trial showings on soybeans on July 26, 2023. 

At 20/20 Seed Labs Inc., we adhere to the ISTA (International Seed Testing Association) standards for vigour and recognize that it is increasingly becoming an important part of the testing requested by both certified seed growers and commercial farmers. 

 The tests were conducted by 20/20 Seed Labs, Winnipeg, a team of experts in soybean quality control. We used routine warm germination and saturated cold vigour tests to screen for the field trial entries. Using the same variety for the entire trial eliminated the possibility of any further variation.  

 The correlation between the laboratory tests and the field trial was extremely accurate. Our overall findings were that the saturated cold tests emulate what happens in the field under a wide variety of field conditions. Given that the seeding conditions were optimal, the performance was not improved due to the poor vigour rating, particularly on the Low and Train Wreck samples.  

 

 Control plot 

“The cornerstone of every seed laboratory test and field analysis.” 
Every trial has a control used as a benchmark. 97%-95% excellent quality for the control. Only a 2% difference in germ versus vigour. Thirty (30) plants m2 meet the expected uniform field emergence.

Plants in control plot   

Control Vigour: Germ – 97%, Vigour – 95%, SPP 2333 

 

High Vigour 

“Textbook high performing seed.” 
High-quality seed and an excellent trial entry with 98%-96% germ and vigour. Both were exceptionally high only a 2% difference in how they performed in testing for optimum and saturated cold conditions—thirty (30) plants m2. 

   

High Vigour:  Germ – 98%, Vigour – 96%, SPP 2410 

 

Moderate Vigour

“Moderate ranking is completely acceptable with good field conditions.”  
The planting value is realized by planting into warm soil temperatures above 12oC. Germ at 97% with vigour at 86% – twenty-six (26) plants m2 with uniform emergence.  

   

Moderate Vigour: Germ – 97%, Vigour – 86%, SPP 2061 

 

Low Vigour

“Use this seed cautiously and adjust the seeding rate according to vigour results.” 
Seeding into warm soil as early as possible can improve the field performance potential of this seed lot. Germ at 91%, with vigour at 77%, and field emergence at 18 plants m2 with open gaps between rows suggests mechanical damage as a factor in the low vigour in testing and uneven emergence in the field.  

   

Low Vigour: Germ – 91%, Vigour – 77%, SPP 2504 

 

Train Wreck

“Pay attention to your seed testing results.” 
Don’t use the germ results independently; always check the vigour. Seed should be handled with care. The germ was 90% and vigour 22%. A huge spread in results suggests extreme seed damage and shows in a poor field performance of 12 plants m2. 

   

Train Wreck (Very Low Vigour): Germ – 90%, Vigour – 22%  

 

This demonstration clearly defined saturated cold tests as a precise measurement for field emergence and uniformity. Only lots with high germination results and moderate to high vigour performed well. Train Wreck had high germination and very low vigour. This test plot had a poor stand with only 12 plants per m2 and clearly illustrated that laboratory germination results, even though completely acceptable, are not enough information for making important seeding decisions. 

The attendees at the Field Day saw firsthand how important knowing vigour is when selecting seed lots. They also asked excellent questions making it a very interactive presentation in the field. It will be very interesting to see how the plots yield at harvest.  

VIDEO:  Soybean Vigour Trial 2023

Video of the field day showcasing the soybean vigour trial at Rutherford Farms, Grosse Isle, Manitoba, featuring Sarah Foster, President 20/20 Seed Labs Inc. and Senior Analyst. Discussion of the plots and the importance of vigour testing, and how the test results present in the field.

June at 20/20 Seed Labs July at 20/20 Seed Labs

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2020 Seed Labs

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support@2020seedlabs.ca

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3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Phone: 1-204-261-3755

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507 – 11 Avenue
Nisku, AB T9E 7N5
Phone: 1-877-420-2099

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  • 1-877-420-2099
  • support@2020seedlabs.ca

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How to Submit a Sample
to 20/20 Seed Labs Inc.

  Please use our our ‘3-Step Process’ when submitting samples to be tested.

Take a Representative Sample

Select Your Testing and Enter Your Sample Details

Ship or Drop Off Your Sample To Our Lab

STEP 1: Take a Representative Sample

We provide testing on seeds, plants and soil. Select the type of sample below that you are submitting for sample collection instructions.

  • Seed Samples

    The table below outlines the type of seed test and required sampling amount needed for each test. Be sure to follow the 4 steps indicated to collect your sample.

    Cereals

    Seed Health & Disease Tests
    500 g (2 1/2 cups)
    Purity Tests
    1 kg (5 cups)

    Pulses

    Seed Health & Disease Tests
    500 g (3 cups)
    Purity Tests
    1 kg (6 cups)

    Grasses & Forages

    All Testing
    250 g

    Canola

    All Testing
    250 g
    20/20 Seed Labs - How to Take a Seed Sample
  • Plant Samples

    The sampling cards below outline the steps to collect your required plant samples for testing.



  • Soil Samples

    The sampling cards below outline the steps to collect your required soil samples for testing.


STEP 2: Select Your Testing and Enter Your Sample Details

Select the testing services you will require from the pricing schedule below, then choose how you want submit your sample: 

Option 1:
Submit your sample online: 

Option 2:
Write Your customer number and sample details on the sample bag and prepare the sample for shipping or drop-off.

STEP 3: Ship or Drop Off Your Sample to Our Lab

Mailing Addresses

NISKU, ALBERTA

20/20 Seed Labs, Inc.
507-11 Avenue
Nisku, AB T9E 7N5
Toll Free: 1-877-420-2099

WINNIPEG, MANITOBA

20/20 Seed Labs, Inc.
3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Toll Free: 1-866-540-7333

After Hours Drop Off

There is a locked and secure after hours drop off box in front of both the Nisku, Alberta and Winnipeg, Manitoba 20/20 Seed Labs offices. Please leave your samples in the drop off box if you are dropping off a sample on the weekends, a statutory holiday, or after 4:30 p.m. on a weekday.

Sample Drop Off Locations

agroplus-logo

Agro Plus Inc.

3895-9th Avenue North
Lethbridge, Alberta
T1H 6G8
Phone: 1-403-327-7070

down-to-earth-labs-logo

Down to Earth Labs

3510-6th Avenue North
Lethbridge, Alberta
T1H 5C3
Phone: 1-403-328-1133

ici-logo

Independent Crop Inputs Inc.

Hwy #3, 2.5km East of Barnwell, Alberta
Box 4690, Taber, Alberta
T1G 2E1
Phone: 1-403-223-1325

  • Step 1: Review Requirements

    Requirements for Submitting Samples

    Please submit the correct sample size for the test required.

    Germination and Vigour Tests
    Most tests of this type require approximately 250 grams or 1 cup of seed. For very large seeds such as some types of peas or beans, up to twice as much seed may be required.

    Disease and Seed Health Tests
    The quantity of seed for most disease testing is the same as a germination test. Some tests with special requirements may require larger amounts of seed.

    Purity Tests
    Test volumes for purity and % pure seed vary with crop type and are available on the purity weight form.

    Rhizobia Test
    Small seeded legumes require a 1 cup sample. Large seeded legumes require a minimum 2 cup sample. Inoculant, loose peat, granules, and liquid  should be tested in the original sealed container size.

    Canadian Seed Institute Samples
    Follow the CSI instructions for the crop type and the weight to be submitted. Example: Wheat, Purity = a minimum of 1 kg of seed.

    APHIS Samples
    Quantities of seed to be tested for an APHIS certificate for export to the US vary with crop type. Please contact us for the correct volume required.

    Soil Samples
    We test soil for clubroot and for a weed check analysis. It is preferable to have a minimum 2 cup or 1 kg sample size.

    Plant Specimens
    Root samples for clubroot analysis should have only one plant per bag and other plant or leaf samples should contain an adequate amount for representation of the tissue. Please pack fresh samples with moist paper towel and drop off at lab or ship by overnight courier only. Pack in a cooler with ice packs if possible. Do not allow sample to contact ice directly.

    FLAX / TRIFFID Tests
    This test requires either 1 or 2 kg of seed. Please CLICK HERE to use the FLAX SAMPLE SUBMISSION FORM for all samples and to confirm the exact amount of seed required.

    If you’re still unsure of the sample size required for your test, please contact us.

  • Step 2: Packaging

    Packaging

    Printed Sample Bags

    20/20 Seed Labs Inc. provides printed sample bags to all of our customers so they may submit samples with all the information clearly noted. As well, you can use packaging or bags from other labs if necessary.

    Special Requirements

    Some samples require special packaging to ensure the sample’s integrity is not compromised during shipping. Please determine if your sample may require any of the following procedures to keep the sample in proper condition.

    Ice Packs

    For samples that need to remain cool during shipping, such as seed or inoculant samples for rhizobia testing.

    Heavy-duty Sealed Poly Bags

    Samples requiring moisture testing should be in a container that will reduce the risk of moisture loss that could cause a change in the sample quality from time of shipping to the time of testing.

  • Step 3: Submit Your Sample

    Guide to Submitting Your Sample Online

    1. Login or sign up for a 20/20 account
    2. Click the “create sample” button
    3. Pick a crop
    4. Choose the number of similar samples for a crop
    5. Enter the information about the seed lot(s); lot numbers, sampler, processor, etc.
    6. Enter the packages or tests you require. (Click here to download a list of our testing packages)
    7. Choose method of printing a bar-coded label (Either a small self-adhesive label or a regular paper label)

    LOG IN NOW | CREATE ACCOUNT

    Other Submission Options

    Submit Your Sample by Mail

    20/20 Seed Labs Inc. receives shipments from all couriers, bus and mail services on a daily basis. You should determine the best method of shipping your samples to ensure it meets your requirements for timeliness and sample integrity upon arrival. We process all samples on arrival and customers with a user name and login can monitor the status of their sample online to determine when it arrived, estimated completion date and check test results.

    Submit Your Sample In-Person

    You may bring your samples directly to our lab, or the after hours drop-off slot. Please call if you have any questions:

    Toll Free: 1-877-420-2099

    In Alberta:
    20/20 Seed Labs Inc.
    507 – 11th Ave
    Nisku, Alberta, Canada
    T9E 7N5

    In Manitoba:
    20/20 Seed Labs Inc.
    3489 Pembina Hwy
    Winnipeg, MB R3V 1A4
    Phone: 1-204-261-3755

X

STEP 3: Ship or Drop Off Your Sample to Our Lab

Mailing Addresses

NISKU, ALBERTA

20/20 Seed Labs, Inc.
507-11 Avenue
Nisku, AB T9E 7N5
Toll Free: 1-877-420-2099

WINNIPEG, MANITOBA

20/20 Seed Labs, Inc.
3489 Pembina Hwy
Winnipeg, MB R3V 1A4
Toll Free: 1-866-540-7333

After Hours Drop Off

There is a locked and secure after hours drop off box in front of both the Nisku, Alberta and Winnipeg, Manitoba 20/20 Seed Labs offices. Please leave your samples in the drop off box if you are dropping off a sample on the weekends, a statutory holiday, or after 4:30 p.m. on a weekday.

Sample Drop Off Locations

agroplus-logo

Agro Plus Inc.

3895-9th Avenue North
Lethbridge, Alberta
T1H 6G8
Phone: 1-403-327-7070

down-to-earth-labs-logo

Down to Earth Labs

3510-6th Avenue North
Lethbridge, Alberta
T1H 5C3
Phone: 1-403-328-1133

ici-logo

Independent Crop Inputs Inc.

Hwy #3, 2.5km East of Barnwell, Alberta
Box 4690, Taber, Alberta
T1G 2E1
Phone: 1-403-223-1325

  • Step 1: Review Requirements

    Requirements for Submitting Samples

    Please submit the correct sample size for the test required.

    Germination and Vigour Tests
    Most tests of this type require approximately 250 grams or 1 cup of seed. For very large seeds such as some types of peas or beans, up to twice as much seed may be required.

    Disease and Seed Health Tests
    The quantity of seed for most disease testing is the same as a germination test. Some tests with special requirements may require larger amounts of seed.

    Purity Tests
    Test volumes for purity and % pure seed vary with crop type and are available on the purity weight form.

    Rhizobia Test
    Small seeded legumes require a 1 cup sample. Large seeded legumes require a minimum 2 cup sample. Inoculant, loose peat, granules, and liquid  should be tested in the original sealed container size.

    Canadian Seed Institute Samples
    Follow the CSI instructions for the crop type and the weight to be submitted. Example: Wheat, Purity = a minimum of 1 kg of seed.

    APHIS Samples
    Quantities of seed to be tested for an APHIS certificate for export to the US vary with crop type. Please contact us for the correct volume required.

    Soil Samples
    We test soil for clubroot and for a weed check analysis. It is preferable to have a minimum 2 cup or 1 kg sample size.

    Plant Specimens
    Root samples for clubroot analysis should have only one plant per bag and other plant or leaf samples should contain an adequate amount for representation of the tissue. Please pack fresh samples with moist paper towel and drop off at lab or ship by overnight courier only. Pack in a cooler with ice packs if possible. Do not allow sample to contact ice directly.

    FLAX / TRIFFID Tests
    This test requires either 1 or 2 kg of seed. Please CLICK HERE to use the FLAX SAMPLE SUBMISSION FORM for all samples and to confirm the exact amount of seed required.

    If you’re still unsure of the sample size required for your test, please contact us.

  • Step 2: Packaging

    Packaging

    Printed Sample Bags

    20/20 Seed Labs Inc. provides printed sample bags to all of our customers so they may submit samples with all the information clearly noted. As well, you can use packaging or bags from other labs if necessary.

    Special Requirements

    Some samples require special packaging to ensure the sample’s integrity is not compromised during shipping. Please determine if your sample may require any of the following procedures to keep the sample in proper condition.

    Ice Packs

    For samples that need to remain cool during shipping, such as seed or inoculant samples for rhizobia testing.

    Heavy-duty Sealed Poly Bags

    Samples requiring moisture testing should be in a container that will reduce the risk of moisture loss that could cause a change in the sample quality from time of shipping to the time of testing.

  • Step 3: Submit Your Sample

    Guide to Submitting Your Sample Online

    1. Login or sign up for a 20/20 account
    2. Click the “create sample” button
    3. Pick a crop
    4. Choose the number of similar samples for a crop
    5. Enter the information about the seed lot(s); lot numbers, sampler, processor, etc.
    6. Enter the packages or tests you require. (Click here to download a list of our testing packages)
    7. Choose method of printing a bar-coded label (Either a small self-adhesive label or a regular paper label)

    LOG IN NOW | CREATE ACCOUNT

    Other Submission Options

    Submit Your Sample by Mail

    20/20 Seed Labs Inc. receives shipments from all couriers, bus and mail services on a daily basis. You should determine the best method of shipping your samples to ensure it meets your requirements for timeliness and sample integrity upon arrival. We process all samples on arrival and customers with a user name and login can monitor the status of their sample online to determine when it arrived, estimated completion date and check test results.

    Submit Your Sample In-Person

    You may bring your samples directly to our lab, or the after hours drop-off slot. Please call if you have any questions:

    Toll Free: 1-877-420-2099

    In Alberta:
    20/20 Seed Labs Inc.
    507 – 11th Ave
    Nisku, Alberta, Canada
    T9E 7N5

    In Manitoba:
    20/20 Seed Labs Inc.
    3489 Pembina Hwy
    Winnipeg, MB R3V 1A4
    Phone: 1-204-261-3755

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